Resources
B-cell epitope mapping
Epitope mapping using libraries of overlapping synthetic peptides is the most efficient way to identify the linear antigenic determinants defined by a particular serum or monoclonal antibody. A convenient way to perform ELISA uses Biotinylated PepSets. The biotin coupled peptides are immobilized on plates coated with avidin or streptavidin and are then probed with antibody.
T-cell epitope mapping
T-cell epitopes are defined as peptide sequences which, in association with proteins on antigen-presenting cells (APC), are required for recognition by specific T-cells. PepSets peptide libraries make it possible to rapidly identify the epitopes of T-Cells, T-Cell clones, T-Helper Cell Clones, T-cell lines, and unpurified T-cells, such as lymph node cells and peripheral blood mononuclear cells (PBMC).
Kinase & Protease Studies
Searching for pharmacologically active peptides or the sites of protein-protein interaction may involve analysing myriads of peptide/protein combinations. Peptide Libraries can be a rich source of candidates for a drug development program. For example, a Structure-Activity Relationship (SAR) study on a bioactive peptide fragment can reveal the critical residue(s) for bioactivity, which can then be further “analoged” to obtain more potent leads and peptidomimetics.