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B-cell epitope mapping

Epitope mapping using libraries of overlapping synthetic peptides is the most efficient way to identify the linear antigenic determinants defined by a particular serum or monoclonal antibody. A convenient way to perform ELISA uses Biotinylated PepSets. The biotin coupled peptides are immobilized on plates coated with avidin or streptavidin and are then probed with antibody.

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