Custom Peptide Specifications
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Procedures utilised by Mimotopes during all stages of manufacture are rigorous.
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Routine Synthetic and Analyical Methods
| Condition or Protocol |
Benefits |
| Synthesis via mild Fmoc chemistry | Well established, safe and reliable method |
| Computer control and monitoring | Ensures accuracy during peptide assembly and purification |
| Crosschecking sequences in database | Identify potential synthetic and solubility issues in advance |
| Purification via automated preparatory HPLC | Enables high-throughput purification processing |
| Fraction assessment via LC/MS | Only the best fractions are selected from the purified peptide |
| Purity assessment via reverse phase HPLC | Purity value is genuine - shoulders are excluded from calculation |
| Identity confirmation via electrospray MS | Non destructive technique to identify the molecular ion |
Supplementary Methods and Options
| Condition or Protocol | Benefits |
| Use of psuedoprolines | Helps break up secondary structure formation where necessary to improve synthesis |
| Ionpairing HPLC | Allows purity determination for highly charged peptides |
| LC/MS assessment for peptides with unusual chromatographic behaviour | Applicable to peptides with particular bond combinations (eg. proline-proline) |
| Preditop analysis | Calculated hydrophobicity value allows useful solubility predictions. Involves small payment prior to ordering peptide |
| Expert pre-sales advice | Guidance with choosing the most appropriate peptide sequence, purity and quantity. Involves small payment prior to ordering peptide |
| After-sales support | Assistance with solubility issues and use within your applications |
Documentation and Packaging
| Condition or Protocol | Benefits |
| Certificate of Analysis (C. of A.) |
Includes details of the peptide, the quantity and purity supplied, and helpful information on the solubility of the peptide in the buffers used during purification |
| Analytical HPLC profile and electrospray MS data | Supplementary verification of peptide integrity |
| Handy technical notes and guidelines | Including: ‘A guide to handling peptides’ and ‘Interpreting the results of peptide analysis’ |
| Packaging under dry nitrogen gas |
Minimizes oxidation or other types of degradation during shipment and storage |